Safety & Environmental Management

Chemical Hygiene Plan

Revised: April, 2000


  1. Accidents and Spills
    1. Eye Contact: Promptly flush eyes with water for a prolonged period (15 minutes) and seek medical attention.
    2. Ingestion: Follow directions on MSDS
    3. Skin Contact: Promptly flush the affected area with water and remove contaminated clothing. If a symptom persists after washing, seek medical attention. Use safety shower if necessary.
    4. Clean-Up: Promptly clean up spills, using appropriate protective apparel and equipment and proper disposal.
  2. Avoidance of Routine Exposure
    1. Use appropriate personal protective equipment.
    2. Do not smell or taste chemicals except as directed by instructor. Vent apparatus which may discharge toxic chemicals (vacuum pumps, distillation columns, etc.) into local exhaust devices.
    3. Inspect gloves and test glove boxes before use.
  3. Choice of Chemicals: Use only those chemicals for which the quality of the available ventilation system is appropriate.
  4. Eating, smoking etc.: Do not eat, smoke, drink, chew gum or apply cosmetics in areas where laboratory chemicals are present; wash hands before conducting these activities.
  5. Equipment and Glassware:
    1. Handle and store laboratory glassware with care to avoid damage. Do not use damaged glassware.
    2. Use extra care with Dewar flasks and other evacuated glass apparatus; shield or wrap them to contain chemicals and fragments should implosion occur. Use equipment only for its designed purpose.
    3. Compressed gas tanks: Handle with care. Do not move without protective cap. Secure tank at all times.
  6. Exiting: Wash areas of exposed skin well before leaving the laboratory.
  7. Horseplay: Avoid practical jokes or other behavior which might confuse startle or distract another worker.
  8. Mouth Suction: Do not use mouth suction for pipeting or starting a siphon.
  9. Personal Apparel: Confine long hair and loose clothing. Wear shoes at all times in the laboratory but do not wear sandals or perforated shoes.
  10. Personal Protection:
    1. See Appendix D for eye protection policy.
    2. Wear appropriate gloves when the potential for contact with toxic materials exists; inspect the gloves before each use, wash them before removal, and replace them periodically.
    3. Use any other protective and emergency apparel and equipment as appropriate.
    4. Remove laboratory coats immediately on significant contamination.
  11. Planning: Seek information and advice about hazards, plan appropriate protective procedures, and plan positioning of equipment before beginning any new operation. This includes reading the Material Safety Data Sheet for substances to be used.
  12. Use of Hood
    1. Use the fume hood for operations which might result in release of toxic vapors or dust. As a rule of thumb, use a hood or other local ventilation device when working with any appreciably volatile substances with a PEL (Permissible Exposure Limit) of less than 50 ppm.
    2. Confirm adequate hood performance before use. Keep materials stored in hoods to a minimum and do not allow them to block vents or air flow.
    3. Leave the hood on when it is not in active use if toxic substances are stored in it or if it is uncertain whether adequate general laboratory ventilation will be maintained when it is off.
  13. Vigilance: Be alert to unsafe conditions and see that they are corrected when detected.
  14. Waste Disposal
    1. Deposit chemical waste in appropriately labeled receptacles and follow all other waste disposal procedures of the Chemical Hygiene Plan.
    2. Refer to the University of Wisconsin Green Bay Hazardous Waste Disposal Guide for information on correct disposal of hazardous waste.
  15. Working Alone: The following policy has been adopted for all persons using NAS and HB facilities:
    1. No one (including faculty/staff) may work alone if activities involve use or manipulation of hazardous materials or if hazardous conditions exist (E.G. high pressure, high voltage, vacuum systems). "Working alone" means working out of sight or sound communications with another person for more than 5 minutes at a stretch. Overnight procedures requiring high pressure, high voltage, or vacuum system are only to be done where there is no other reasonable way of accomplishing this procedure and exposure to any potential hazard is minimal. Situations where there is danger of explosion, implosion or exposure to UV, X-ray or high voltage require a notice posted on door if left unattended.
    2. Undergraduate students in scheduled courses must be supervised at all times. Students may not work out of hours for any scheduled course unless specific permission is granted by the faculty person supervising the lab and students will not be doing any manipulations using hazardous chemicals and hazardous conditions do not exist. If Barb Troedel or Mark Damie will be asked to give students access, the supervising faculty/staff person must submit a list of students, course number, location, and dates students have permission to have access to areas. This list must be submitted at the beginning of each semester. If a written list is not submitted, students will only have access through the supervising faculty/staff.
    3. Student researchers (both graduate and undergraduate students) may never work alone if working with hazardous chemicals or if hazardous conditions exist. Ultimately it is the responsibility of the Principal Investigator to ensure safe supervision of undergraduate and graduate researchers.

The following is a list of standard operating procedures which could be made available to students either as a part of the laboratory manual or during student training.


  1. No eating, drinking or smoking is permitted in any laboratory.
  2. Eye protection must be worn when required by the lab instructor. Contact lenses are not advisable, even under splash goggles.
  3. Wear sensible clothing. Loose fitting clothing and open sandals or open footwear should not be worn.
  4. Long hair must be confined.
  5. No unauthorized experimentation is allowed. Do not change written laboratory procedures without permission of the instructor.
  6. Students may not work in the laboratory without an instructor present. (Exceptions may be made depending on the course.)
  7. Students are not allowed to work in instructional laboratories outside of regularly scheduled hours without written permission of instructor.
  8. Students should know locations of all available safety equipment. This includes eye wash stations, safety showers, fire extinguishers, fire blanket and first aid supplies.
  9. Pipetting by mouth is not allowed. Never place anything in the mouth except as directed by instructor while in the laboratory. Smell chemicals only by wafting a small amount of vapor toward the nose with the hand.
  10. Personal protective equipment besides eye protection (eye protection should be worn at all times) should be used at the direction of the laboratory instructor. This equipment includes gloves when working with certain corrosives and organic solvents and laboratory aprons.
  11. Keep lab bench clear of book bags and outer clothing. These should be placed in areas provided. Students are responsible for maintaining a safe and clean work area.
  12. Laboratory fume hoods should be used for all operations which have the potential to release fumes, gases or volatile solvent vapors in excess of recommended exposure levels. Follow written laboratory procedures and the laboratory instructor's directions. Notify the instructor if you think the fume hood is not functioning properly.
  13. Read lab procedures before entering the laboratory. Do not proceed with an experiment if you do not understand the procedure. All chemical names and identities should be carefully double-checked prior to any use. Check labeling before using a chemical so that potential hazards are know.
  14. Report all injuries, no matter how minor, to the laboratory instructor. The instructor will give guidance on any appropriate treatment which may be needed or call Security if necessary.
  15. Proper disposal of laboratory waste is essential. Do not dispose of any chemical down sewer/sink without approval from the instructor. Use appropriate waste containers when provided.
  16. Clean up spills promptly. If you should break a mercury thermometer, notify the instructor so that the mercury is promptly recovered. If you have questions on spill clean-up, ask your instructor.
  17. Only students registered for the class are allowed into the laboratory.
  18. Students should clean work area and wash hands thoroughly before leaving the laboratory.
I have read and I understand the above standard operating procedures. I understand that it is my responsibility to follow the above procedures and I agree to follow these procedures

Date: _________________       Signature: _______________________________________